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. 2016 Nov 18;291(53):27289–27297. doi: 10.1074/jbc.M116.744672

FIGURE 4.

FIGURE 4.

Siah2 acts upstream of BMP-4 to promote adipogenesis. A and B, Bmp-4, Wisp2, and Zfp423 gene expression was assayed in wild-type and Siah2KO inguinal adipose tissue (A) or prior to induction of adipogenesis in wild-type and Siah2KO primary inguinal adherent stromal cells (−MDI) and on day 4 post induction (+MDI) (B). C, markers of adipogenesis (Pparg, Fabp4, and Lpl), Wnt10b, Wisp2, and Zfp423 gene expression were assayed during adipogenesis in the absence or presence of 40 ng/ml BMP-4. D, Oil Red O staining of neutral lipid accumulation on day 4 post-induction of adipogenesis in wild-type and Siah2KO stromal cells. Siah2KO stromal cells were incubated in the absence (Siah2KO/−BMP-4) or presence of 40 ng/ml BMP-4 (Siah2KO/+BMP-4). Statistical significance of Siah2KO was compared with the wild type (A), wild-type −MDI (B), or the corresponding day 0 (C). #, p < 0.05; *, p < 0.01; ∧, p < 0.01 compared with −MDI, day 0.