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. 2016 Nov 8;291(52):26686–26697. doi: 10.1074/jbc.M116.757732

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© 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — Regulation of IL6 and NOS2 promoters by hypertonicity and TonEBP is unique in NP cells. A and B, schematics showing IL6 and NOS2 promoter luciferase constructs and their predicted TonE. C and D, treatment of NP cells with hypertonic medium has no effect on IL6 promoter activity (C) and decreases NOS2 promoter activity (D). Under hypertonic conditions, expression of DN-TonEBP suppresses activity levels of both IL6 (C) and NOS2 (D) reporters. E and F, under isotonic conditions, expression of DN-TonEBP suppresses activity of the IL6 promoter only slightly (E), but has no effect on the NOS2 promoter (F). G and H, activity levels of both IL6 (G) and NOS2 (H) promoter reporters are suppressed by overexpression of TonEBP. Quantitative measurements represent mean ± S.E. of ≥3 biological replicates and 3 technical replicates per biological replicate. *, p ≤ 0.05; **, p ≤ 0.01; ***, p ≤ 0.001; ****, p ≤ 0.0001. NS, not significant.