FIGURE 7.

Ets-2 and NFAT do not interact physically. A, Jurkat cells were cultured in CM or P/I, harvested, and subjected to immunoprecipitation (IP) with a human anti-Ets-2 antibody. Detection of interactions of Ets-2 with NFAT-1 or NFAT-2 was carried out by Western blotting analysis with the corresponding specific antibodies. B, as a positive control, extracts from the same cells were immunoprecipitated with a human anti-NFAT antibody for the detection of the interaction of NFAT with c-Fos by Western blotting analysis. An anti-human IgG antibody was used as a negative control to confirm the specificity of the immunoprecipitations.