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Global simulation for the stopped-flow fluorescence for formation of PRMT1-SAH-H4 (A and B), PRMT1-SAH-H4me1 (C and D), and PRMT1-SAH-H4me2a (E and F) ternary complexes. The final concentrations are 0.4 μm for PRMT1, 50 μm for SAH (in A, C, and E), 10 μm for H4 and H4me2a (in B and F), and 1.5 μm for H4me1 (D). Solid lines are the fitted curves from a global simulation based on the model shown in Fig. 10.
