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Global simulation for stopped-flow fluorescence for the formation of PRMT1-SAM-H4me2a (A and B), PRMT1-SAM-H4 (C and D), and PRMT1-SAM-H4me1 (E and F) ternary complexes. The final concentrations are 0.4 μm for PRMT1, 400 μm for SAM in (A, C, and E), 10 μm for H4 and H4me2a in (B and D), and 1.5 μm for H4me1 (F). Solid lines are the fitted curves from a global simulation based on the model shown in Fig. 10.
