SCHEME 1.

Proposed detailed kinetic model of overall process for the PRMT1-catalyzed H4 peptide methylation. A, first methylation event. B, second methylation event. C and D, formation of dead-end complex. Specific values are given for the rate constants that are well constrained. In the cases where rate constants are not constrained, the boundaries are shown. Blue-colored values are the best fitted results from the global simulation (but not constrained). The rate constants of the chemical reaction were determined in our previous work (33).