TABLE 2.
Parameters of binary binding between enzyme (E) and ligand (L, including SAH or H4me1 peptide)
k1app, k−1app, Kd1app, and fluorescence change were yielded from a global simulation of each set of signal traces using the one-step model (E + L ↔ EL) with KinTek software.
| E | L | k1app (range) | k−1app (range) | Kd1app | Fluorescence change |
|---|---|---|---|---|---|
| s−1μm−1 | s−1 | μm | % | ||
| wt | SAH | 0.68 (0.64–0.72) | 0.33 (0.29–0.37) | 0.49 | −13 |
| W145F | SAH | 0.39 (0.3–0.39) | 0.38 (0.35–0.38) | 0.97 | −26 |
| W294A | SAH | 0.58 (0.46–0.73) | 0.29 (0.23–0.38) | 0.5 | −3 |
| wt | H4me1 | 79 (63–99) | 19 (16–22) | 0.24 | 42 |
| W145F | H4me1 | 60 (57–62) | 44 (38–50) | 0.73 | 69 |
| W294A | H4me1 | 74 (55–103) | 3.9 (1.0–7.9) | 0.05 | 8 |