FIGURE 7.

FPPS recovered from viperin-expressing cells is un-modified. A, native-PAGE analysis of FPPS purified from HEK293T cells. Lane 1 standard proteins (native molecular masses in kDa indicated on the left); lane 2, FPPS expressed in the absence of viperin; lane 3, FPPS co-expressed with viperin. B, activity of FPPS purified from HEK293T cells in absence and in presence of co-expressed viperin; the small difference in rates is not considered significant. C and D, LC-MS of FPPS purified from HEK293T cells in the absence (C) and presence of co-expressed viperin (D). No significant differences in the spectra are apparent.