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. 2016 Oct 31;291(52):26816–26836. doi: 10.1074/jbc.M116.719823

FIGURE 2.

FIGURE 2.

Ace-induced Isc and ICl response to CFTR inhibitor and classical CaCC inhibitors NPPB and NFA and ANO inhibitors AO1 and TAO1 in Caco-2 cells. A, representative trace showing the effects of FSK (10 μm) and CFTR inhibitor CFTRinh-172 (20 μm) on Ace-stimulated Isc. CFTRinh-172 inhibited FSK- but not Ace-stimulated Isc in Caco-2 cells (inset). Values are means ± S.E.; n = 3–6. B, effects of cumulative addition of CaCC blockers DIDS (500 μm), TFN (10 μm), AO1 (10 μm), and TAO1 (10 μm) on Ace-induced Isc. AO1 was most effective to inhibit Ace-induced Isc than cumulative addition of different CaCC blockers. C, summary of the inhibitory effects on ICl by various CaCC inhibitors NPPB (100 μm), NFA (100 μm), AO1 (10 μm), TAO1 (10 μm), TFN (10 μm), and TA (100 μm) in Caco-2 cell monolayers. D, mouse ileum. Values are means ± S.E.; n = 5–8. Error bars represent S.E. *, p < 0.01, significant inhibition by blockers (ANOVA). NS, statistically not significant; ND, not determined.