FIGURE 4.

ERK1/2 physiologically regulates PGC-1α expression and protein in mouse kidney. A, representative immunoblot of phosphorylated ERK1/2 after treatment with trametinib in mouse kidney cortex. p, phosphorylated; MW, molecular weight. B, representative immunoblot of PGC-1α and TFAM proteins after treatment with trametinib in the kidney cortex. The black line represents the splicing of the blot from the same membrane. C, mRNA expression of PGC-1α, NRF1, TFAM, and COX 1 following trametinib administration at 4 h. D and E, densitometry analysis of PGC-1α (D) and TFAM (E) protein at 4 h following trametinib. Data are represented as mean ± S.E., n ≥ 5. Different superscripts indicate statistically significant differences (p < 0.05).