FIGURE 10.

Reversibility of AgTRPA1 heat responses. The heat responses of AgTRPA1 with and without its N-terminal ARD (Δ1–766 AgTRPA1) were reversible when continuously exposed to different temperatures (30–35-min total recording time). Shown are representative recordings from 2–3 separate experiments. Black traces are shown at the same time scale (1 s), whereas orange traces show part of the recordings at a higher time resolution (30 and 100 ms). Purified AgTRPA1 (temperatures 30, 40, 30, and 25 °C) and Δ1–766 AgTRPA1 (temperatures 30, 25, 15, and 40 °C) were inserted into planar lipid bilayers, and channel currents were recorded with the patch clamp technique in a symmetrical K⁺ solution at a test potential of +60 mV (c, closed channel state; upward deflections, open channel state).