Fig. 1.
The A. fumigatus ΔschA mutant is more sensitive to osmotic stress and increased calcium concentrations.
A and B. Radial Growth of the A. fumigatus wild-type, ΔschA, and ΔschA::schA+ on MM, MM + CaCl2 500 mM or MM + MnCl2 1 mM. The data are expressed as radial growth sorbitol/radial growth control (mm).
C. The strains were inoculated in liquid MM with increasing concentrations of sorbitol or NaCl and incubated with agitation for 48 h at 37°C.
The data are expressed as dry weight sorbitol/dry weight control (g). The radial diameter and dry weight data are expressed as average ± standard deviation of three independent biological repetitions (* and *** denote p < 0.01 and 0.001, respectively, by t-ests when compared to the wild-type strain).
D and E. Western blot analysis for the total protein level and phosphorylation state of Rps6. The wild-type and ΔschA strains were grown for 16 h at 37°C and exposed, or not, to rapamycin or osmotic stress. Proteins were normalized by Ponceau red staining. Signal intensities were quantified using the Image J software by dividing the intensity of SakA-P/SakA ratio and expressed as fold increase from the control (0 min).