Table 4.

Proteins identified as more expressed in the ΔschA mutant strain upon growth on YPD medium (time 0 h) and transfer to 1.0 M Sorbitol (for 2 or 4 h). For the full list refer to Supplementary Tables S3–S5.

Aspergillus fumigatus		ΔschA × Wild type(t-test difference)
Strain Af293	Strain A1163	Protein annotation	0 h	2 h	4 h
oxidative and osmotic stresses
AFUA_3G02270	AFUB_046060	mycelial catalase Cat1	1.505	NIa	NI
AFUA_6G12450	AFUB_078460	awh11. Putative heat shock protein	NI	NI	2.200
AFUA_3G14540	AFUB_034690	heat shock protein Hsp30/Hsp42	NI	1.068	NI
AFUA_6G12180	AFUB_078180	DprB. Fungal dehydrin-like protein	NI	6.192	1.394
AFUA_7G04520	AFUB_090060	DprC. Dehydrin-like protein	NI	NI	1.149
AFUA_7G02070	AFUB_088630	AIF-Iike mitochondrial oxidoreductase; conidia- enriched protein	NI	4.063	1.332
AFUA_8G01090	AFUB_085510	Putative thioredoxin; hypoxia repressed protein	NI	NI	1.459
AFUA_1G09890	AFUB_009330	Protein with Yap1-dependent induction in response to hydrogen peroxide	NI	1.341	NI
AFUA_4G14380	AFUB_071650	Glutathione S-transferase. Putative	NI	NI	1.176
AFUA_2G05060	AFUB_022090	Alternative oxidase, mediates the cyanide-insensitive respiratory pathway	NI	1.290	NI
AFUA_5G07000	AFUB_054560	Putative NAD binding Rossmann fold oxidoreductase	NI	NI	1.625
AFUA_1 G02090	AFUB_002470	predicted oxidoreductase activity	NI	1.413	NI
AFUA_3G00330	AFUB_048120	NAD dependent epimerase/dehydratase family protein	NI	NI	1.911
AFUA_5G09910	AFUB_057500	nitroreductase family protein	NI	2.686	NI
AFUA_4G09220	AFUB_066340	flavin-binding monooxygenase-like protein	NI	NI	1.128
AFUA_8G04340	AFUB_083200	Cystathionine gamma-lyase	NI	1.160	NI
AFUA_8G06080	AFUB_081400	Putative flavohemoprotein	NI	NI	1.633
AFUA_1 G09930	AFUB_009370	glycerol dehydrogenase; protein level decreases upon heat shock	NI	NI	1.040
Protein synthesis
AFUA_5G05450	AFUB_053010	40S ribosomal protein S3Ae; 40S ribosomal protein S1	NI	1.953	NI
AFUA_4G07845	AFUB_064950	Ortholog(s) have cytosolic large ribosomal subunit	NI	2.226	NI
AFUA_6G02440	AFUB_095900	60s ribosomal protein L24	NI	1.354	NI
AFUA_7G05290	AFUB_090870	Cytosolic small ribosomal subunit S13/S15	NI	1.813	NI
AFUA_6G12720	AFUB_078720	40S ribosomal protein S29	NI	1.191	NI
AFUA_3G06840	AFUB_042210	Putative cytosolic small ribosomal subunit S4	NI	1.102	NI
AFUA_2G10300	AFUB_026110	40S ribosomal protein S17	NI	1.541	NI
AFUA_2G07970	AFUB_023990	60S ribosomal protein L19	NI	1.618	NI
AFUA_2G08130	AFUB_024140	Ortholog(s) have cytosolic large ribosomal subunit localization	NI	1.829	NI
AFUA_1G17120	AFUB_016510	Elongation factor 1 gamma	NI	2.034	NI
AFUA_3G06640	AFUB_042410	40S ribosomal protein S27	NI	1.688	NI
AFUA_2G03380	AFUB_020450	Ortholog(s) have cytosolic large ribosomal subunit	NI	2.383	NI
AFUA_1G06770	AFUB_007150	40S ribosomal protein S26	NI	1.851	NI
AFUA_2G11850	AFUB_027590	Allergenic ribosomal L3 protein	NI	1.510	NI
AFUA_1G09440	AFUB_008890	40S ribosomal protein S23	NI	2.711	NI
AFUA_1G16523	AFUAJ g16523	cytosolic small ribosomal subunit localization	NI	1.201	NI
AFUA_2G10440	AFUB_026240	Ortholog(s) have mRNA binding, small ribosomal subunit rRNA binding	NI	1.975	NI
Chromosome metabolism
AFUA_2G14080	AFUB_029700	chromosome segregation protein SudA	NI	NI	1.435
AFUA_2G16080	AFUB_031760	role in mitotic sister chromatid cohesion and mating- type region	2.503	NI	NI
AFUA_3G14260	AFUB_034970	mismatched base pair and cruciform DNA recognition protein	NI	3.679	NI
AFUA_3G10480	AFUB_038680	meiotic sister chromatid recombination protein Ish1/ Msc1	NI	1.129	NI
Phosphatidic acid metibolism
AFUA_3G12330	AFUB_036830	Putative phosphatidyl synthase	NI	NI	1.896
AFUA_7G05580	AFUB_091160	Putative phospholipase D. pldA	NI	1.270	NI
AFUA_4G12000	AFUB_068990	phosphatidylinositol phospholipase C	1.089	NI	NI
AFUA_4G11720	AFUB_068730	Putative phosphatidyl synthase	NI	1.452	NI
Carbohydrate metabolism
AFUA_2G15430	AFUB_031090	Sorbitol/xylulose reductase	NI	1.566	NI
AFUA_6G07720	AFUB_073680	Phosphoenolpyruvate carboxykinase AcuF.	1.233	NI	NI
AFUA_6G03540	AFUB_094750	Malate synthase AcuE	NI	NI	1.156
Miscellaneous
AFUA_2G04610	AFUB_021660	Role in post translational protein targeting to mem- brane and TRC complex	1.204	NI	1.543
AFUA_2G08920	AFUB_024830	GDSL Lipase/Acylhydrolase family protein	NI	NI	1.066
AFUA_2G08920	AFUB_031880	Uracil phosphoribosyltransferase	NI	NI	1.008
Afu2G16530	AFUB_032210	Cyanate hydratase	1.041	NI	NI
AFUA_3G07410	AFUB_041670	Putative isoamyl alcohol oxidase	NI	NI	1.138
AFUA_3G06660	AFUB_042390	Putaive NIPSNAP family protein	NI	NI	1.528
AFUA_3G00650	AFUB_047780	Lap2. Putative aminopeptidase Y	NI	NI	1.508
AFUA_5G02330	AFUB_050860	Major allergen and cytotoxin ΔspF1	NI	NI	1.168
AFUA_5G12590	AFUB_060250	Solid-state culture expressed protein (Aos23)	NI	NI	1.365
AFUA_4G05900	AFUB_062990	Transcript up-regulated in conidia exposed to neutro- phils (2)	NI	NI	1.298
AFUA_4G08240	AFUB_065340	Putative zinc-containing alcohol dehydrogenase; conidia-enriched protein	NI	1.185	NI
AFUA_6G07590	AFUB_073550	Has domain(s) with predicted zinc ion binding activity	NI	1.634	NI
AFUA_6G07880	AFUB_073860	DUF500 and SH3 domain protein	1.072	NI	NI
AFUA_6G08750	AFUB_074710	PrncI, role in hyphal growth and cytosol. mitochond- rion localization	NI	NI	1.313
AFUA_6G11430	AFUB_077440	AldA. Putative aldehyde dehydrogenase	1.003	NI	1.068
AFUA_8G05580	AFUB_081980	Putative coenzyme A transferase, coaT	NI	1.728	NI
AFUA_8G01930	AFUB_084680	Methyltransferase LaeA-like	1.183	NI	1.020
AFUA_8G00550	AFUB_086020	Putative methyl transferase; member of the pseurotin A gene cluster	2.742	NI	NI
AFUA_7G01000	AFUB_087580	Putative alcohol dehydrogenase involved in ethanol metabolism	1.659	NI	NI
AFUA_7G01340	AFUB_087920	Putative RPEL repeat protein	NI	NI	1.315
AFUA_7G06050	AFUB_091630	Ortholog(s) have SNARE binding, polyubiquitin bind- ing activity	NI	1.158	NI
AFUA_6G04920	AFUB_093370	Putative NAD-dependent formate dehydrogenase, fdh	NI	2.031	NI
AFUA_4G04318	AFUB_098700	Copper resistance protein Crd2. similar to Cu-binding metallothionein	NI	2.265	NI
AFUA_3G07150	AFUB_041900	Succinate-semialdehyde dehydrogenase	1.141	NI	NI
AFUA_6G13330	AFUB_001440	Putative RNA binding protein of unknown function	NI	1.150	NI
Unknown
AFUA_1G06350	AFUB_006730	Uncharacterized	NI	NI	1.301
AFUA_3G05610	AFUB_043380	Uncharacterized	NI	NI	1.020
AFUA_6G02535	AFUB_095800	Uncharacterized	NI	1.861	1.498
AFUA_4G02840	AFUB_100280	Uncharacterized	NI	3.513	NI
AFUA_3G02430	AFUA_3g02430	Uncharacterized	NI	NI	2.177
AFUA_8G05650	AFUB_081900	Hypothetical protein	NI	NI	1.784
AFUA_5G14890	AFUB_079000	Hypothetical protein	1.683	NI	1.298
AFUA_6G11850	AFUB_077850	Protein of unknown function; hypoxia induced protein	NI	1.908	NI
AFUA_5G13100	AFUB_060810	Hypothetical protein	NI	3.732	NI
AFUA_3G09990	AFUB_039180	Hypothetical protein	NI	1.452	NI
AFUA_1G13550	AFUB_013040	Uncharacterized	NI	3.024	NI
AFUA_1G15260	AFUB_014810	Uncharacterized	NI	NI	1.785
AFUA_1G08960	AFUB_008380	Uncharacterized	NI	NI	1.242

^aNI = not identified, the protein was not identified in this timepoint.