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. 2016 Oct 13;291(49):25339–25350. doi: 10.1074/jbc.M116.740795

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© 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — Influence of T3lec on human core 1 glycan biosynthesis in vivo. HeLa cells were transfected with mock vector, ppGalNAc-T3 (T3), or catalytically inactive mutant ppGalNAc-T3^D277H (T3^D277H). Effects of mock vector (green), ppGalNAc-T3 (light blue), and ppGalNAc-T3^D277H (red) overexpression on core 1 glycan expression were evaluated by flow cytometry of cells stained with biotinylated PNA (A) or ABL (C). Fluorescence intensity values of FITC (expressed in arbitrary units (AU)) correspond to experimental conditions with PNA (B) or ABL (D). Bars represent means of three independent experiments, and error bars represent S.D. *, p < 0.05.