FIGURE 6.

Shrm SD2 can directly activate Rock1 kinase. A, magnetic nickel-nitrilotriacetic acid resin coated with full-length (FL) Rock1 were incubated in a kinase assay containing MYPT. Samples were analyzed by Western blotting using antibodies to pMYPT and Rock1. B, the effect of the Shrm2 SD2 variant L1501A on Rock activation. pMYPT levels were normalized to Rock1 enzyme signal and Rock1 activity was compared with the Rock1 alone basal level and reported as the mean with error bars representing the standard deviation from five independent replicates. A student t-test was used to calculate p-values with statistically significant values indicated with an asterisk.