FIGURE 2.
Recombinant apo-PfPKA-R inhibits PKA-mediated phosphorylation. A, illustration of the ELISA-based phosphorylation assay using the recombinant tail of AMA1, a validated PfPKA target. B, native phosphorylation activity of PfPKA-C (indicated by A405 nm) can be efficiently inhibited by addition of recombinant apo-PfPKA-R in a concentration-dependent manner. This effect can be efficiently reversed by addition of supplementary cAMP and is due specifically to the apo-PfPKA-R as the inhibitory effect of the fragment is lost upon heat denaturation (heat-inactivated). Each data point and whiskers are the mean/standard deviation of two technical replicates. Graph shown is representative of three independent biological replicates.