TABLE 2.
Isothermal calorimetry binding constants
| Construct | Ligand | N | Kda | ΔH | ΔS |
|---|---|---|---|---|---|
| nm | kcal mol−1 | cal mol−1 K−1 | |||
| PfPKAR.141.441 | cAMP | 2.1 ± 0.1 | 1.2 ± 0.2 | −18.9 ± 0.6 | −22 ± 2 |
| PfPKAR.141.441 | (Sp)-8-Br-cAMPS | 1.2 ± 0.1 | 1300 ± 850 | −10.5 ± 2 | −8.1 ± 1.1 |
| PfPKAR.141.441 | (Sp)-2-Cl-cAMPS | 2.1 ± 0.1 | 8.9 ± 4 | −11.9 ± 0.4 | −3 ± 2 |
| PfPKAR.297.441 | cAMP | 0.9 ± 0.1 | 6.0 ± 0.6 | −11.1 ± 0.1 | −0.2 ± 0.3 |
| PfPKAR.297.441 | (Sp)-8-Br-cAMPS | ND | ND | ND | |
| PfPKAR.297.441 | (Sp)-2-Cl-cAMPS | 0.9 ± 0.1 | 9.1 ± 11 | −7.1 ± 0.1 | 11.7 ± 0.9 |
a Data were determined at 295 K; ND, none determined.