A, Western blotting of flagellin extractions of RT027 wild type and flagellin modification mutants. Lane M, marker (prestained protein marker; Fermentas); lane 1, R20291; lane 2, R20291fliC; lane 3, GT1; lane 4, GT2; lane 5, GT3; lane 6, CDR20291_0245; lane 7, CDR20291_0246; lane 8, GT1comp. B, schematic diagram illustrates the structure of flagellin type B modification and the contribution of each GT. Flagellin type B is modified with one N-acetylglucosamine (white square) linked to two rhamnoses (white circles) (with or without a methyl group (Me)). White hexagon, terminal peptidyl-sugar moiety.