FIGURE 6.

TLR5 activation in RT027 and corresponding mutants. Glycosylation moieties on C. difficile flagellin do not contribute to TLR5 activation. Untransfected (HEK-WT) and TLR5-transfected (HEK-TLR5) HEK cells were co-cultured with WT R20291 (A) or CD1426 (B) and their respective flagellin mutants at a multiplicity of infection of 10. IL-8 protein was quantified 8 h post-infection. Data are presented as the mean ± S.E. from three independent experiments with three technical replicates each. *, p < 0.05; **, p < 0.01; ***, p < 0.001.