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. 2016 Oct 25;291(49):25591–25607. doi: 10.1074/jbc.M116.761296

FIGURE 6.

FIGURE 6.

Overexpression 4.1R or myogenin has no effect on normal C2C12 differentiation. FLAG-4.1R or T7-myogenin introduced into C2C12 was grown in differentiation medium for the indicated number of days and analyzed. A, expression of FLAG-4.1R. Anti-4.1R Ab detects both endogenous and exogenous 4.1R. Tubulin served as a loading control. B, expression of T7-myogenin. Anti-myogenin Ab detects both endogenous and exogenous myogenin. Anti-T7 Ab detects exogenously expressed myogenin. Tubulin served as a loading control. C, overexpression of 4.1R or myogenin had no effect on normal C2C12 differentiation. 4.1R- or myogenin-transfected C2C12 cells were grown for 3 days in differentiation medium. Differentiation and fusion indices were obtained from three independent experiments. Each experiment was repeated three times, and S.D. values (error bars) were determined.