FIGURE 6.
27-mer(I860A) inhibits Gαq-mediated neuronal depolarization in the prefrontal cortex of mice. A, pretreatment of a representative prefrontal cortex neuron with 27-mer(L859E+I860A) had no effect on action potential firing in response to CCh (10 μm) (top panel). In contrast, depolarization was lost when 27-mer(I860A) was used (center panel) or the M1 receptor antagonist pirenzepine (2 μm) was applied with 27-mer(L859E+I860A) (bottom panel). B, the inward current induced by carbachol after indicated pretreatments. Sample sizes included six neurons for the active and inactive peptides and four neurons for the active peptide with agonist. *, p < 0.05 from baseline. **, p < 0.05 between the different experiments n.s., not significant.