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. 2016 Oct 25;291(49):25656–25666. doi: 10.1074/jbc.M116.753145

FIGURE 3.

FIGURE 3.

IFNγ increases CD38 expression in AML cells. AML cell lines MOLM-13, MV4-11, OCI-AML3, and THP-1 (n = 3 or more separate experiments each) and primary AML apheresis samples were incubated with or without 10 ng/ml IFNγ for 18 h (qPCR) or for 24 h (flow cytometry). A and B, CD38 expression in AML cell lines (A) and primary AML apheresis samples (B, n = 7 donors) was measured by qPCR. C and D, CD38 expression in AML cell lines (C) and primary AML apheresis samples (D, n = 8 donors, representative histogram shown; inset bar graph depicts all donors) was measured by flow cytometry. E, primary AML apheresis samples (n = 4 donors) were treated for 18 h with concentrations of IFNγ from 0 to 10 ng/ml. qPCR was done to measure transcript levels of CD38. *, p ≤ 0.05 compared with untreated (UT). Error bars, S.D.