FIGURE 3.

Characterization of the TRPP3 other intracellular or extracellular cysteine residues and prediction of candidate palmitoylation residues. A, schematic illustration of all intracellular or extracellular cysteine residues in human TRPP3. B, averaged Ca²⁺-activated currents obtained at −50 mV from oocytes expressing TRPP3 WT or an indicated mutant. Currents were averaged from three independent experiments with indicated total numbers of tested oocytes. ** indicates p < 0.01. C, amino acid sequence alignment of TRPP3 N-terminal fragments containing Cys-38 or the corresponding cysteine from indicated species. National Center for Biotechnology Information accession number for sequences used here are as follows: NP_057196 (human); NP_852087 (mouse); NP_001099822 (rat); XP_001168415 (monkey); XP_012999134 (pig); XP_002718661 (rabbit); XP_006938169 (cat); and XP_005637930 (dog). Potential phosphorylation sites near the cysteine residue are marked with ●. D, palmitoylation scores for cysteine residues in the human TRPP3 N terminus predicted by a CSS-palm version 4.0 algorithm. A higher score value indicates a higher probability.