FIGURE 8.

Relationship between palmitoylation at Cys-38 and phosphorylation at Thr-39. A, averaged Ca²⁺-activated currents obtained at −50 mV from oocytes expressing TRPP3 WT without or with 20 μm 2BP treatment (WT+2BP), C38A, T39A, without or with 20 μm 2BP treatment (T39A+2BP), or C38A/T39A (double C38A and T39A mutations). Currents were averaged from three independent experiments with indicated total numbers of tested oocytes and normalized to that of TRPP3 WT. ** and *** indicate p < 0.01 and 0.001, respectively. B, representative whole-mount immunofluorescence data showing the PM expression of TRPP3 WT, C38A/T39A, C38A/T39E, and T39A with 20 μm 2BP treatment (T39A+2BP). C, phosphorylation of TRPP3NT WT and TRPP3NT with the T39A, C38A, or S41A mutation in HEK cells. D, averaged and normalized currents obtained at −50 mV from oocytes expressing TRPP3 WT, C38A, T39E, or C38A/T39E. Currents were averaged from three independent experiments with indicated total numbers of tested oocytes and normalized to that of TRPP3 WT. ** indicates p < 0.01. E, representative confocal images showing subcellular localization of EGFP, EGFP-TRPP3CT, EGFP-TRPP3NT, and EGFP-TRPP3NT T39E in HEK cells. Scale bar, 10 μm.