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. 2016 Oct 24;291(49):25692–25705. doi: 10.1074/jbc.M116.745471

FIGURE 10.

FIGURE 10.

Zebrafish foxo3b suppresses hypoxia-induced gene expression through vhl. A, ectopic expression of foxo3b by mRNA injection suppressed vegf expression in wild-type sibling embryos (vhl+/+) but not in vhl-null embryos (vhl−/−), as revealed by semiquantitative real-time RT-PCR assays. GFP mRNA injections were used as controls. B, ectopic expression of foxo3b by mRNA injection suppressed ldha expression in the wild-type sibling embryos (vhl+/+) but not in vhl-null embryos (vhl−/−), as revealed by semiquantitative real-time RT-PCR assays. GFP mRNA injections were used as controls. C, ectopic expression of foxo3b by mRNA injection suppressed cited2 expression in wild-type sibling embryos (vhl+/+) but not in vhl-null embryos (vhl−/−), as revealed by semiquantitative real-time RT-PCR assays. GFP mRNA injections were used as controls. D, ectopic expression of foxo3b by mRNA injection suppressed il11a expression in wild-type sibling embryos (vhl+/+) but not in vhl-null embryos (vhl−/−), as revealed by semiquantitative real-time RT-PCR assays. GFP mRNA injections were used as controls. E, expression of myc-tagged foxo3b mRNA injected into embryos was confirmed by Western blotting assays. F, expression of control GFP mRNA injected into embryos was examined under a fluorescence stereomicroscope (Leica M205).