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. 2016 Oct 24;291(49):25692–25705. doi: 10.1074/jbc.M116.745471

FIGURE 3.

FIGURE 3.

Knockdown of FOXO3a causes VHL expression to be reduced. A, knockdown of FOXO3a by two sets of FOXO3a shRNA in HEK293T cells was confirmed by semiquantitative real-time RT-PCR assays. Luciferase shRNA was used as a control. B, knockdown of FOXO3a in HEK293T caused VHL expression to be reduced. C, knockdown of FOXO3a by two sets of FOXO3a shRNA in H1299 cells was confirmed by semiquantitative real-time RT-PCR assays. Luciferase shRNA was used as a control. D, knockdown of FOXO3a in H1299 cells caused VHL expression to be reduced. E, knockdown of FOXO3a resulted in reduction of endogenous pVHL and induction of endogenous HIF-1α in HEK293T cells, as confirmed by Western blotting assays. F, knockdown of FOXO3a resulted in reduction of endogenous pVHL and induction of endogenous HIF-1α in H1299 cells, as confirmed by Western blotting assays.