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. 2016 Oct 24;291(49):25692–25705. doi: 10.1074/jbc.M116.745471

FIGURE 4.

FIGURE 4.

FOXO3a suppresses hypoxia-inducible gene expression via VHL. A, overexpression of FOXO3a caused SLC2A1 expression to be reduced in HEK293T cells under normoxia and hypoxia (2% O2). B, overexpression of FOXO3a caused LDHA expression to be reduced in HEK293T cells under normoxia and hypoxia (2% O2). C, knockdown of FOXO3a caused SLC2A1 expression to be increased in H1299 cells under normoxia and hypoxia (2% O2). D, knockdown of FOXO3a caused LDHA expression to be increased in H1299 cells under normoxia and hypoxia (2% O2). E, knockdown of endogenous VHL by transient transfection with two sets of VHL shRNA was confirmed by Western blotting assays. F, when VHL was knocked down, the suppressive effect of FOXO3a on SLC2A1 expression was abrogated under normoxia. G, knockdown of VHL abolished the suppressive effect of FOXO3a on SLC2A1 expression under hypoxia.