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. 2016 Oct 24;291(49):25692–25705. doi: 10.1074/jbc.M116.745471

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© 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — The expression pattern of vhl in zebrafish and the effect of foxo3b on vhl promoter activity. A and B, semiquantitative real-time RT-PCR assays demonstrated the expression pattern of foxo3b (A) and vhl (B) in different tissues of adult zebrafish. C, zebrafish vhl promoter activity was induced after injection of the foxo3b expression vector in embryos. D, injection of a foxo3b-3A mutant induced zebrafish vhl promoter activity more dramatically (third column versus second column). The vhl promoter was not activated after injection of a foxo3b dominant-negative mutant (dn-foxo3b, p = 0.089).