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. 2016 Oct 24;291(49):25692–25705. doi: 10.1074/jbc.M116.745471

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© 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 7. — Zebrafish foxo3b enhances vhl expression. A, ectopic expression of foxo3b by mRNA injection induced vhl expression in embryos, as detected by WISH. GFP mRNA was used as a control. B, ectopic expression of foxo3b by mRNA injection enhanced vhl expression in embryos, as revealed by semiquantitative real-time RT-PCR assays. GFP mRNA was used as a control. C, knockdown of foxo3b in embryos by morpholino oligonucleotide injection reduced vhl expression. a, standard morpholino oligonucleotide control (STD-MO, 8 ng/individual embryo). b, foxo3b translation-blocking (ATG code) morpholino oligonucleotide (foxo3b-ATG-MO, 8 ng/individual embryo). c, foxo3b splicing-blocking morpholino oligonucleotide (foxo3b-sp-MO, 8 ng/individual embryo). D, injection of dominant-negative foxo3b mRNA (dn-foxo3b) in zebrafish embryos reduced vhl expression (b) compared with those injected with GFP mRNA (a).