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. 2016 Oct 24;291(49):25692–25705. doi: 10.1074/jbc.M116.745471

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© 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 8. — Generation of foxo3b^−/− zebrafish via CRISPR/Cas9 technology. A, targeting strategy for generating mutations in exon 2 of foxo3b. The sequence difference in foxo3b between the mutants and their wild-type siblings is indicated. B, verification of zebrafish foxo3b disruption by HMA. Genomic DNA was prepared from the mutants and their wild-type siblings. C, the predicted protein products of foxo3b in the mutants and their wild-type siblings. TAD, transactivation domain. D and E, expression of foxo3b (D) or vhl (E) in wild-type (foxo3b^+/+) and foxo3b-null(foxo3b^−/−) zebrafish embryos under normoxia and hypoxia.