Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Oct 26;291(49):25716–25728. doi: 10.1074/jbc.M116.748624

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

FIGURE 5. — TβRIII interacts with Wnt through its GAG chains. A, OVCA429 cells transiently expressing full-length TβRIII were immunoprecipitated (IP) and analyzed for Wnt3a-TβRIII interactions using the Wnt pulldown assay described under “Experimental Procedures” and previously (13). All values were normalized to the IgG sample. B, conditioned medium from COS-7 cells transiently expressing full-length TβRIII and Wnt3a-HA was immunoprecipitated using anti-TβRIII and immunoblotted using anti-HA and anti-TβRIII to analyze Wnt3a-TβRIII interactions as described under “Experimental Procedures” and previously (21, 27, 77). C, OVCA429 cells transiently expressing GFP (control), full-length TβRIII, or TβRIII-ΔGAG were immunoprecipitated using anti-TβRIII and immunoblotted (IB) using anti-Wnt3a and anti-TβRIII to analyze Wnt3a-TβRIII interactions using the Wnt pulldown assay described under “Experimental Procedures” and previously (13) All values were normalized to the GFP sample, and all figures represent at least two independent biological trials.