TABLE 4.
Potency (pEC50) and maximal response (Emax), for cAMP production at the CLR co-expressed with each RAMP, stimulated with various agonists measured in HEK-293S cells in the presence or absence of pertussis toxin
Data are the mean ± S.E. of n individual data sets. No statistical difference was found between untreated and PTX-treated HEK-293S cells using Student's t test.
| Untreated |
Treated |
|||||
|---|---|---|---|---|---|---|
| pEC50a | Emaxb | n | pEC50a | Emaxb | n | |
| RAMP1 | ||||||
| CGRP | 9.88 ± 0.1 | 59.98 ± 1.1 | 5 | 9.87 ± 0.1 | 72.92 ± 2.3 | 5 |
| AM | 8.13 ± 0.1 | 60.00 ± 3.1 | 5 | 8.03 ± 0.1 | 61.26 ± 2.6 | 5 |
| AM2 | 8.74 ± 0.1 | 68.94 ± 1.2 | 5 | 8.78 ± 0.1 | 68.30 ± 1.6 | 5 |
| RAMP2 | ||||||
| CGRP | 8.00 ± 0.1 | 32.56 ± 1.0 | 5 | 7.88 ± 0.1 | 39.32 ± 1.7 | 5 |
| AM | 9.39 ± 0.1 | 30.34 ± 0.8 | 5 | 9.38 ± 0.1 | 33.28 ± 1.2 | 5 |
| AM2 | 8.57 ± 0.1 | 40.30 ± 1.5 | 5 | 8.58 ± 0.2 | 30.52 ± 2.0 | 5 |
| RAMP3 | ||||||
| CGRP | 8.42 ± 0.1 | 40.84 ± 0.6 | 5 | 8.38 ± 0.1 | 39.84 ± 1.0 | 5 |
| AM | 9.63 ± 0.1 | 39.09 ± 1.2 | 5 | 9.49 ± 0.2 | 42.26 ± 1.6 | 5 |
| AM2 | 8.01 ± 0.1 | 33.75 ± 1.5 | 5 | 7.79 ± 0.2 | 28.21 ± 2.4 | 5 |
a The negative logarithm of the agonist concentration required to produce a half-maximal response.
b The maximal response to the ligand expressed as a percentage of the maximal cAMP production as determined using 100 μm forskolin stimulation in the presence of PTX treatment.