Figure 4. The Nanos NED interacts with subunits of the deadenylase and decapping complexes.

• A–F
  Western blots showing the interaction of GFP‐tagged Dm Nanos (either full length, NED, or ΔNED) and the indicated HA‐tagged proteins. The co‐immunoprecipitations were performed using a polyclonal anti‐GFP antibody. GFP‐tagged firefly luciferase (F‐Luc) served as a negative control. Inputs and immunoprecipitates were analyzed using anti‐GFP and anti‐HA antibodies. For the GFP‐tagged proteins, 3% of the inputs and 10% of the immunoprecipitates were loaded, whereas for the HA‐tagged proteins, 1% of the input and 30% of the immunoprecipitates were analyzed. In all panels, cell lysates were treated with RNase A prior to immunoprecipitation.

Source data are available online for this figure.