Table 1.
Data collection and refinement statistics
| NOT123–Nanos | NOT123–Nanos SeMet | NOT123 apo | |
|---|---|---|---|
| Space group | P 21 | P 21 | P 21 |
| Unit cell | |||
| dimensions a, b, c (Å) | 76.0, 135.7, 105.0 | 77.1, 135.8, 105.8 | 76.0, 135.3, 101.3 |
| angles α, β, γ (°) | 90, 108, 90 | 90, 108, 90 | 90, 108, 90 |
| Data collection a | |||
| Wavelength (Å) | 1.00005 | 0.97866 | 0.99982 |
| Resolution range (Å) | 50–3.1 (3.17–3.10) | 50–3.9 (4.00–3.90) | 50–2.9 (2.98–2.90) |
| R sym (%) | 7.5 (74.1) | 20.7 (81.3) | 7.5 (63.3) |
| Completeness (%) | 99.6 (98.2) | 99.7 (98.1) | 99.4 (99.7) |
| Mean I/σ(I) | 13.0 (1.7) | 8.6 (2.4) | 11.9 (2.1) |
| Unique reflections | 36,658 (2,706) | 18,985 (1,369) | 43,109 (3,191) |
| Multiplicity | 3.45 (3.45) | 6.92 (6.78) | 3.26 (3.27) |
| Refinement | |||
| R cryst (%) | 16.5 | 18.1 | |
| R free (%) | 22.7 | 22.1 | |
| Coordinate error (Å) | 0.42 | 0.35 | |
| Number of atoms | |||
| All atoms | 14,548 | 14,109 | |
| Protein | 14,548 | 14,109 | |
| Water | 0 | 0 | |
| Average B factor (Å2) | |||
| All atoms | 99.3 | 83.9 | |
| Ramachandran plot | |||
| Favored regions (%) | 95.3 | 95.8 | |
| Disallowed regions (%) | 0.3 | 0.4 | |
| RMSD from ideal geometry | |||
| Bond lengths (Å) | 0.010 | 0.010 | |
| Bond angles (°) | 1.10 | 1.10 | |
a
Values in parentheses are for highest resolution shell.