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. 2016 Dec 23;9:9–19. doi: 10.2147/BCTT.S124860

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© 2017 Cook et al. This work is published and licensed by Dove Medical Press Limited

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LU inhibits VEGF secretion but not VEGF mRNA expression in MDA-MB-231 (4175) LM2 cells.

Notes: (A) Cells were treated at 37°C for 16 hours with 10, 50, or 100 µM LU or vehicle control. Levels of VEGF in supernatants were measured by ELISA. Data represent mean ± SEM (n = 4). ***Significantly different compared with controls (P < 0.001, ANOVA). **Significantly different compared with controls and either 10 or 50 μM LU (P < 0.001, ANOVA). (B) Cells were treated for 6 hours with 10, 25, or 50 µM LU or vehicle control, after which total mRNA was isolated and RT-PCR for VEGF isoforms performed. Upper panel shows PCR-amplified VEGF bands for VEGF₁₈₉, VEGF₁₆₅, and VEGF₁₂₁, together with a GAPDH band for normalization. Bar graph (bottom) represents mean band intensities (VEGF/GAPDH) ± SEM (n = 3). Results represent values from three independent experiments. No statistically significant differences were observed compared with controls (P = 0.149, ANOVA).

Abbreviations: ANOVA, analysis of variance; ELISA, enzyme-linked immunosorbent assay; LU, luteolin; mRNA, messenger RNA; RT-PCR, reverse transcription-polymerase chain reaction; SEM, standard error of the mean; VEGF, vascular endothelial growth factor.