Figure 7.
LU induces apoptosis in MDA-MB-231 (4175) LM2 cells in vitro.
Notes: Cells were treated with 10, 25, or 50 µM LU or vehicle control for 24 hours, then stained with AV and PI, and analyzed by FACS. Bars represent mean (AV+ + PI+) ± SEM (n = 4). *Significantly different compared with controls; **significantly different compared with controls and 10 μM LU; and ***significantly different compared with controls, 10 µM LU, and 25 µM LU (P < 0.001, ANOVA).
Abbreviations: ANOVA, analysis of variance; AV, Annexin V; FACS, fluorescence-activated cell sorting; LU, luteolin; PI, propidium iodide; SEM, standard error of the mean.