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Detection of cell death in zebrafish embryos using acridine orange.
Notes: (A) Posterior trunk and tail of 48 and 72 hpf (a and b) vehicle control embryos in 1% DMSO and (c and d) treated embryos in 20 μg/mL of girinimbine. White arrows indicate dead cells. The magnification is ×10. (B) Quantification data of the number of dead cells in the tail. *Significant difference (P<0.05) compared with control (n=30).
Abbreviations: hpf, hours postfertilization; DMSO, dimethyl sulfoxide.
