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. 2016 Dec 28;11:103–121. doi: 10.2147/DDDT.S115135

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© 2017 Iman et al. This work is published and licensed by Dove Medical Press Limited

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Detection of apoptotic cell death in zebrafish embryos using TUNEL assay.

Notes: (A) Posterior trunk and tail of 48 and 72 hpf (a and b) vehicle control embryos in 1% DMSO and (c and d) treated embryos in 20 μg/mL of girinimbine. Black spots indicate TUNEL+ response (apoptotic signaling), and black arrows represent apoptotic cells. The magnification is ×10. (B) Quantification data of the number of apoptotic cells in the tail. *Significant difference (P<0.05) compared with the control (n=30).

Abbreviations: TUNEL, terminal deoxynucleotidyl transferase dUTP nick end labeling; hpf, hours postfertilization; DMSO, dimethyl sulfoxide.