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. 2016 Sep 14;13(12):1286–1299. doi: 10.1080/15476286.2016.1232238

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© 2016 The Author(s). Published with license by Taylor & Francis Group, LLC

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 4. — dis3L2 knockdown largely affects transcripts expressed at low levels. (A) Kernel density plot comparing the overall gene expression profiles of the 2 parental controls (UAS = ;UAS-dis3L2^RNAi; , GAL4 = ;;69B-GAL4). (B) Kernel density plot comparing dis3L2 knockdown (Dis3L2^KD = ;UAS-dis3L2^RNAi/+ ; 69B-GAL4/+) to the grouped control (Control =;UAS-dis3L2^RNAi; and ; 69B-GAL4). Knockdown of dis3L2 results in a general shift in expression of the transcripts normally expressed at low levels to a level of higher expression. The moderately and highly expressed transcripts show less variation. (C) Scatter plots showing the fold changes between control and Dis3L2^KD samples. All detected transcripts are shown in the top panel (black) while consistently misexpressed transcripts red are represented in the bottom panel. Dashed lines represent ± 2 fold changes whilst dot-dashed lines represent ± 10 fold changes.