TESCO 5’ sgRNA preparation |
TESCO-dist-F |
Cas9 cleavage target site 41 bp 3' to the 5' end of TESCO |
CACCGTTGGAGTTCCGATTTAGAC
|
TESCO-dist-R |
AAACGTCTAAATCGGAACTCCAAC |
TESCO-dist-F-T7 |
TAATACGACTCACTATAGGGGTTGGAGTTCCGATTTAGAC
|
TESCO 5’ sgRNA preparation |
TESCO-prox-F |
Cas9 cleavage target site 8 bp 3’ to 3’ end of TESCO |
CACCGTCTGTTAACGTATGACACGA
|
TESCO-prox-R |
AAACTCGTGTCATACGTTAACAGAC |
TESCO-prox-F-T7 |
TAATACGACTCACTATAGGGGTCTGTTAACGTATGACACGA
|
sgRNA preparation |
sgRNA-uni.R |
pX330 plasmid |
AAAAGCACCGACTCGGTGCC |
TES 5’ sgRNA preparation |
TES-dist-F |
Cas9 cleavage target site 25 bp 5' to the 5' end of TES |
CACCGAGGTGTGTGGAAGCGGGCA
|
TES-dist-R |
AAACTGCCCGCTTCCACACACCTC |
TES 3’ sgRNA preparation |
TES prox-F |
Cas9 cleavage target site 20 bp 3’ to 3’ end of TES |
CACCGGGAACATTGGCAGGGCCCT
|
TES-prox-R |
AAACAGGGCCCTGCCAATGTTCCC |