. 2017 Jan 3;13(1):e1006520. doi: 10.1371/journal.pgen.1006520

Table 1. Primers for CRISPR genome editing.

Application	Primer name	Location	Sequence 5' to 3' (20-mer sgRNA recognition sequences in bold)
TESCO 5’ sgRNA preparation	TESCO-dist-F	Cas9 cleavage target site 41 bp 3' to the 5' end of TESCO	CACCGTTGGAGTTCCGATTTAGAC
	TESCO-dist-R		AAACGTCTAAATCGGAACTCCAAC
	TESCO-dist-F-T7		TAATACGACTCACTATAGGGGTTGGAGTTCCGATTTAGAC
TESCO 5’ sgRNA preparation	TESCO-prox-F	Cas9 cleavage target site 8 bp 3’ to 3’ end of TESCO	CACCGTCTGTTAACGTATGACACGA
	TESCO-prox-R		AAACTCGTGTCATACGTTAACAGAC
	TESCO-prox-F-T7		TAATACGACTCACTATAGGGGTCTGTTAACGTATGACACGA
sgRNA preparation	sgRNA-uni.R	pX330 plasmid	AAAAGCACCGACTCGGTGCC
TES 5’ sgRNA preparation	TES-dist-F	Cas9 cleavage target site 25 bp 5' to the 5' end of TES	CACCGAGGTGTGTGGAAGCGGGCA
TES 5’ sgRNA preparation	TES-dist-R	Cas9 cleavage target site 25 bp 5' to the 5' end of TES	AAACTGCCCGCTTCCACACACCTC
TES 3’ sgRNA preparation	TES prox-F	Cas9 cleavage target site 20 bp 3’ to 3’ end of TES	CACCGGGAACATTGGCAGGGCCCT
TES 3’ sgRNA preparation	TES-prox-R	Cas9 cleavage target site 20 bp 3’ to 3’ end of TES	AAACAGGGCCCTGCCAATGTTCCC