Table 3. Primers for Real-time quantitative RT-PCR.
| Primer name | Gene | Sequence 5' to 3' |
|---|---|---|
| AQ- Sox9-F | Sox9 | AGTACCCGCATCTGCACAAC |
| AQ- Sox9-R | TACTTGTAATCGGGGTGGTCT | |
| AQ- Foxl2-F | Foxl2 | AGGGAGAGAATAAAACATTCATGG |
| AQ- Foxl2-R | GCAAACTCCAAGGCCATTAC | |
| AQ- Amh-F | Amh | CGAGCTCTTGCTGAAGTTCCA |
| AQ- Amh-R | GAAGTCCACGGTTAGCACCAA | |
| AQ- Sox8-F | Sox8 | GCAAGACCCTAGGCAAGCTGT |
| AQ- Sox8-R | TCTGGGTGGTCTTTCTTGTGC | |
| AQ- Wnt4-F | Wnt4 | CTGGACTCCCTCCCTGTCTTT |
| AQ-Wnt4-R | CATGCCCTTGTCACTGCAA | |
| AQ- Tbp-F | Tbp | ACGGACAACTGCGTTGATTTT |
| AQ- Tbp-R | ACTTAGCTGGGAAGCCCAAC | |
| AQ- Hprt1-F | Hprt1 | TCCTCCTCAGACCGCTTT |
| AQ- Hprt1-R | CCTGGTTCATCATCGCTAATC | |
| NG- Sox9-F | Sox9 | AAGAAAGACCACCCCGATTACA |
| NG- Sox9-R | CAGCGCCTTGAAGATAGCATT | |
| NG- Foxl2-F | Foxl2 | CGGCATCTACCAGTACATCATAGC |
| NG- Foxl2-R | GCACTCGTTGAGGCTGAGGTTG | |
| NG- Amh-F | Amh | CCACGGTTAGCACCAAATAGC |
| NG- Amh-R | CACACAGAACCTCTGCCCTACTC | |
| NG- Sox8-F | Sox8 | AGCGAGAAGAGGCCGTTTG |
| NG- Sox8-R | TCAGTACCAGAGTCTGAGTCG | |
| NG- Sry-F | Sry | GCTGGGATGCAGGTGGAAAA |
| NG- Sry-R | CCCTCCGATGAGGCTGATATT | |
| NG- Hprt1-F | Hprt1 | GCTTGCTGGTGAAAAGGACCTCTCGAAG |
| NG- Hprt1-R | CCCTGAAGTACTCATTATAGTCAAGGGCAT |