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. 2017 Jan 3;12(1):e0169294. doi: 10.1371/journal.pone.0169294

Fig 6. ABA-induced DNA breaks and repair activity in wild-type and DSB-related mutants.

Fig 6

(A) Two-week-old wild-type and DSB-related mutant seedlings were treated with 30 μM ABA for 24 h at room temperature and the extent of DSB induction was analysed by the neutral (N/N) comet assays. The extent of DNA damage is represented by the fraction of DNA migrating in the comet tail. (B) DSB repair during various post-treatment recovery periods, including 0 h, 4 h, 8 h and 12 h, respectively. Additional data sets for 2 h and 6 h of post-treatment recovery periods are available on request. Mean percentage of DNA in the comet tails of wild-type and other mutant lines at various recovery time points under standard growth conditions. Maximum damage is normalized as 100% at t = 0 for all lines. Data shown are means (±SD) of three independent replications.