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. 2017 Jan 3;12(1):e0168395. doi: 10.1371/journal.pone.0168395

Fig 4. Analysis of DNA double-strand breaks foci.

Fig 4

Cells were subjected or not to IUdR pretreatment and then exposed to 4 Gy irradiation with 137Cs or RX200 (72.5 keV) conditions. Immunostaining of 53BP1 and IUdR was performed 1 h post irradiation. (A) An example of immunostaining of radiation-induced 53BP1 foci (second column) and IUdR (third column), counterstaining with Dapi (first column) and merge are also shown. (B) An example of automated counting. Range distribution of foci per cell was determined in F98 (C) and in SF763 (D) cells (n = 3).