Skip to main content
. 2017 Jan 3;12(1):e0167871. doi: 10.1371/journal.pone.0167871

Table 1. Antigenicity index (Ai) prediction of HBsAg with Jameson-Wolf algorithm within amino acids 110–160.

HBsAg W1S (wt) W3S (wt) W1S P120K W1S T123D W1S F134V W1S P120K/T123D W1S Q129R/G145R W1S N146G
Positions Residues Ai Residues Ai Residues Ai Residues Ai Residues Ai Residues Ai Residues Ai Residues Ai
110 Leu -0.05 Leu -0.05 Leu -0.05 Leu -0.05 Leu -0.05 Leu -0.05 Leu -0.05 Leu -0.05
111 Pro -0.05 Pro -0.05 Pro -0.05 Pro -0.05 Pro -0.05 Pro -0.05 Pro -0.05 Pro -0.05
112 Gly 0.35 Gly 0.35 Gly 0.35 Gly 0.35 Gly 0.35 Gly 0.35 Gly 0.35 Gly 0.35
113 Thr 0.4 Thr 0.4 Thr 0.4 Thr 0.4 Thr 0.4 Thr 0.4 Thr 0.4 Thr 0.4
114 Ser 0.4 Ser 0 Ser 0 Ser 0.4 Ser 0.4 Ser 0 Ser 0.4 Ser 0.4
115 Thr 0.4 Thr 0 Thr 0 Thr 0.4 Thr 0.4 Thr 0 Thr 0.4 Thr 0.4
116 Thr 0.65 Thr 0.34 Thr 0.34 Thr 0.4 Thr 0.65 Thr 0.34 Thr 0.65 Thr 0.65
117 Ser 1.3 Ser 2.08 Ser 2.08 Ser 1.11 Ser 1.3 Ser 2.08 Ser 1.3 Ser 1.3
118 Thr 1.2 Thr 2.27 Thr 2.27 Thr 1.07 Thr 1.2 Thr 2.27 Thr 1.2 Thr 1.2
119 Gly 2.05 Gly 2.91 Gly 2.91 Gly 1.78 Gly 2.05 Gly 2.91 Gly 2.05 Gly 2.05
120 Pro 2.5 Lys 3.4 Lys 3.4 Pro 2.59 Pro 2.5 Lys 3.4 Pro 2.5 Pro 2.5
121 Cys 2.25 Cys 2.91 Cys 2.61 Cys 3.1 Cys 2.25 Cys 2.91 Cys 2.25 Cys 2.25
122 Lys 2 Lys 2.57 Lys 2.27 Lys 2.79 Lys 2 Lys 2.57 Lys 1.6 Lys 2
123 Thr 0.35 Asp 1.83 Thr 1.53 Asp 1.78 Thr 0.35 Asp 1.83 Thr 0.35 Thr 0.35
124 Cys -0.05 Cys 1.04 Cys 0.44 Cys 1.32 Cys -0.05 Cys 1.04 Cys -0.05 Cys -0.05
125 Thr -0.3 Thr 0.3 Thr -0.3 Thr 0.61 Thr -0.3 Thr 0.3 Thr -0.3 Thr -0.3
126 Ile -0.6 Ile -0.3 Ile -0.6 Ile -0.3 Ile -0.6 Ile -0.3 Ile -0.05 Ile -0.6
127 Pro -0.6 Pro -0.6 Pro -0.6 Pro -0.6 Pro -0.6 Pro -0.6 Pro 0.6 Pro -0.6
128 Ala 0.35 Ala 0.35 Ala 0.35 Ala 0.35 Ala 0.35 Ala 0.35 Ala 1.4 Ala 0.35
129 Gln 0.8 Gln 0.8 Gln 0.8 Gln 0.8 Gln 0.8 Gln 0.8 Arg 2.4 Gln 0.8
130 Gly 0.65 Gly 0.65 Gly 0.65 Gly 0.65 Gly 0.65 Gly 0.65 Gly 2.5 Gly 0.65
131 Thr 0.35 Thr 0.35 Thr 0.35 Thr 0.35 Thr 0.35 Thr 0.35 Thr 1.45 Thr 0.35
132 Ser 0.15 Ser 0.45 Ser 0.15 Ser 0.15 Ser 0.45 Ser 0.15 Ser 1.2 Ser 0.15
133 Met 0 Met -0.1 Met 0 Met 0 Met -0.1 Met 0 Met 0.5 Met 0
134 Phe -0.2 Val -0.2 Phe -0.2 Phe -0.2 Val -0.2 Phe -0.2 Phe 0.05 Phe -0.2
135 Pro 0.2 Pro -0.2 Pro 0.2 Pro 0.2 Pro -0.2 Pro 0.2 Pro 0.2 Pro 0.2
136 Ser 0.2 Ser 0.2 Ser 0.2 Ser 0.2 Ser 0.2 Ser 0.2 Ser 0.2 Ser 0.2
137 Cys 0.2 Cys -0.2 Cys 0.2 Cys 0.2 Cys -0.2 Cys 0.2 Cys 0.2 Cys 0.2
138 Cys 0.64 Cys 0.24 Cys 0.64 Cys 0.64 Cys 0.24 Cys 0.64 Cys 0.64 Cys 0.64
139 Cys 0.98 Cys 0.98 Cys 0.98 Cys 0.98 Cys 0.98 Cys 0.98 Cys 1.18 Cys 0.98
140 Thr 1.47 Thr 1.07 Thr 1.47 Thr 1.47 Thr 1.07 Thr 1.47 Thr 1.67 Thr 1.47
141 Lys 2.46 Lys 2.46 Lys 2.46 Lys 2.46 Lys 2.46 Lys 2.46 Lys 3.06 Lys 2.46
142 Pro 3.4 Pro 3.4 Pro 3.4 Pro 3.4 Pro 3.4 Pro 3.4 Pro 3.4 Pro 3.4
143 Ser 3.06 Ser 3.06 Ser 3.06 Ser 3.06 Ser 3.06 Ser 3.06 Ser 2.86 Ser 3.06
144 Asp 2.57 Asp 2.57 Asp 2.57 Asp 2.57 Asp 2.57 Asp 2.57 Asp 2.72 Asp 2.57
145 Gly 1.93 Gly 1.93 Gly 1.93 Gly 1.93 Gly 1.93 Gly 1.93 Arg 2.38 Gly 1.93
146 Asn 1.19 Asn 1.19 Asn 1.19 Asn 1.19 Asn 1.19 Asn 1.19 Asn 1.74 Gly 1.19
147 Cys -0.3 Cys -0.3 Cys -0.3 Cys -0.3 Cys -0.3 Cys -0.3 Cys 1.1 Cys -0.3
148 Thr -0.6 Thr -0.6 Thr -0.6 Thr -0.6 Thr -0.6 Thr -0.6 Thr -0.3 Thr -0.6
149 Cys -0.6 Cys -0.6 Cys -0.6 Cys -0.6 Cys -0.6 Cys -0.6 Cys -0.6 Cys -0.6
150 Ile -0.6 Ile -0.6 Ile -0.6 Ile -0.6 Ile -0.6 Ile -0.6 Ile -0.53 Ile -0.6
151 Pro -0.6 Pro -0.6 Pro -0.6 Pro -0.6 Pro -0.6 Pro -0.6 Pro -0.46 Pro -0.6
152 Ile -0.45 Ile -0.45 Ile -0.45 Ile -0.45 Ile -0.45 Ile -0.45 Ile -0.24 Ile -0.45
153 Pro -0.05 Pro -0.05 Pro -0.05 Pro -0.05 Pro -0.05 Pro -0.05 Pro 0.23 Pro 0.15
154 Ser 0.35 Ser 0.35 Ser 0.35 Ser 0.35 Ser 0.35 Ser 0.35 Ser 0.7 Ser 0.35
155 Ser 0.35 Ser 0.35 Ser 0.35 Ser 0.35 Ser 0.35 Ser 0.35 Ser 0.63 Ser 0.35
156 Trp -0.2 Trp -0.2 Trp -0.2 Trp -0.2 Trp -0.2 Trp -0.2 Trp 0.01 Trp -0.2
157 Ala -0.6 Ala -0.6 Ala -0.6 Ala -0.6 Ala -0.6 Ala -0.6 Ala -0.46 Ala -0.6
158 Phe -0.6 Phe -0.6 Phe -0.6 Phe -0.6 Phe -0.6 Phe -0.6 Phe -0.53 Phe -0.6
159 Ala -0.6 Ala -0.6 Ala -0.6 Ala -0.6 Ala -0.6 Ala -0.6 Ala -0.6 Ala -0.6
160 Arg -0.6 Arg -0.6 Arg -0.6 Arg -0.6 Arg -0.6 Arg -0.6 Arg -0.6 Arg -0.6

The Jameson-Wolf antigenicity index (Ai) of HBsAs was predicted by using the Protean application of Lasergene 13 (DNASTAR Inc., Madison, WI). Mutation-induced changes in the Ai of affected upstream and downstream amino acid residues are indicated in bold and italics.