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. 2017 Jan 3;12(1):e0168944. doi: 10.1371/journal.pone.0168944

Fig 5. Effect of different component preincubation orders on HPU (A) and JBU (B) inactivated by Pal.

Fig 5

The initial 20 min preincubation mixture contained components given in brackets. Further preincubation was continued for another 20 min after the addition of the last component (given outside the brackets). The concentrations of both ureases were 0.25 mg/mL. The concentration of all thiols used was 1.25 mM and Pal was 0.5 mM. Residual activity values were expressed as percent of the control activity. Results summarized here are the mean value of n = 3 ± SD. The residual activity of urease in the presence of Pal without the thiols is given for comparison. *P < 0.05 and **P < 0.01 were considered as significant differences when compared with control value (urease in the presence of Pal without the protector), and #P < 0.05 and ##P < 0.01 in comparison to the control (the first column of each group) and, as determined by one-way ANOVA.