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. 2017 Jan 3;12(1):e0169229. doi: 10.1371/journal.pone.0169229

Fig 2. NVP-AEW541 induces autophagy in MDA-MB-231 and BT-549 cells.

Fig 2

(A) NVP-AEW541 induced a significant accumulation of LC3-II and Beclin-1 and down-regulated p62 in MDA-MB-231 and BT-549 cells, as measured by western blotting. One representative from three independent experiments is shown. (B and C) Immunostaining and fluorescence microscopy analysis of LC3 (green) and the nucleus (blue) demonstrated that NVP-AEW541 promoted autophagy, as indicated by increased LC3-II puncta. Results were obtained from four independent experiments, and data are presented as the mean ± SD, *p<0.05, **p<0.01, ***p<0.001.