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. 2017 Jan 3;12(1):e0169229. doi: 10.1371/journal.pone.0169229

Fig 3. 3-MA inhibits NVP-AEW541-induced autophagy and enhances anti-proliferative effects of NVP-AEW541 in MDA-MB-231 and BT-549 cells.

Fig 3

(A) 3-MA inhibited the autophagy induced by NVP-AEW541 in MDA-MB-231 and BT-549 cells, as shown by western blotting. One representative from three independent experiments is shown. (B-D) 3-MA combined with NVP-AEW541 significantly suppressed the proliferation of MDA-MB-231 and BT-549 cells compared with NVP-AEW541 alone, as detected by EdU (B and C) and CCK-8 assays (D). Results were obtained from at least four independent experiments, and data are presented as the mean ± SD, *p<0.05, **p<0.01, ***p<0.001.