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. 2017 Jan 3;12(1):e0169229. doi: 10.1371/journal.pone.0169229

Fig 5. 3-MA and Atg7 siRNA increase the level of apoptosis in MDA-MB-231 and BT-549 cells.

Fig 5

(A) 3-MA and Atg7 siRNA decreased the protein level of Bcl-2 and increased that of Bax in MDA-MB-231 and BT-549 cells, as shown by western blotting. (B) 3-MA and Atg7 siRNA markedly increased the level of apoptosis induced by NVP-AEW541 in MDA-MB-231 cells, as determined by Annexin V/PI dual staining. (C) 3-MA and Atg7 siRNA markedly increased the level of apoptosis induced by NVP-AEW541 in BT-549 cells, as determined by Annexin V/PI dual staining. All experiments were repeated three times, and data are presented as the mean ± SD, *p<0.05, **p<0.01, ***p<0.001.