Table 2.
Comparison of the amino acid compositions of the actually synthesized DNA libraries (the full length library) with the target composition
| Library name | Natural | No Cys | c-Fos e', g'- | |||
| Target | Actual | Target | Actual | Target | Actual | |
| Ala | 8.9% | 11.4% | 5.3% | 6.7% | 0.0% | 0.0% |
| Arg | 4.7 | 3.5 | 5.3 | 5.9 | 30.0 | 29.7 |
| Asn | 4.4 | 3.5 | 5.3 | 4.3 | 0.0 | 0.0 |
| Asp | 5.5 | 8.6 | 5.3 | 5.9 | 0.0 | 0.0 |
| Cys | 2.8 | 2.5 | 0.0 | 0.0 | 0.0 | 0.0 |
| Gln | 3.9 | 2.1 | 5.3 | 5.5 | 20.0 | 19.6 |
| Glu | 6.2 | 6.9 | 5.3 | 5.5 | 20.0 | 25.0 |
| Gly | 7.8 | 12.0 | 5.3 | 8.2 | 0.0 | 0.0 |
| His | 2.0 | 1.2 | 5.3 | 2.7 | 0.0 | 0.0 |
| Ile | 4.6 | 3.9 | 5.3 | 5.9 | 0.0 | 0.0 |
| Leu | 7.5 | 3.7 | 5.3 | 4.3 | 0.0 | 0.0 |
| Lys | 7.0 | 6.2 | 5.3 | 4.7 | 30.0 | 27.9 |
| Met | 1.7 | 1.1 | 5.3 | 3.5 | 0.0 | 0.0 |
| Phe | 3.5 | 4.0 | 5.3 | 5.1 | 0.0 | 0.0 |
| Pro | 4.6 | 2.5 | 5.3 | 2.4 | 0.0 | 0.0 |
| Ser | 7.1 | 8.1 | 5.3 | 1.6 | 0.0 | 0.0 |
| Thr | 6.0 | 5.8 | 5.3 | 6.7 | 0.0 | 0.0 |
| Trp | 1.1 | 1.8 | 5.3 | 8.2 | 0.0 | 0.0 |
| Tyr | 3.5 | 4.4 | 5.3 | 4.7 | 0.0 | 0.0 |
| Val | 6.9 | 6.9 | 5.3 | 8.2 | 0.0 | 0.0 |
| Term | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 |
| Number of sequenced codon | - | 568 | - | 255 | - | 219 |
| Correlation coefficient | - | 0.85 | - | 0.66 | - | 1.00 |
| Sum of absolute errors | - | 26.9% | - | 28.1% | - | 5.66% |
"Term" denotes stop codons. In each target composition, [Term] = 0. "Natural": the library with the natural abundance (the average composition in 207 natural proteins) [18]; "No Cys": the library with uniform composition except [Cys] = 0; "c-Fos e', g'-": a c-Fos mutant library containing only 4 kinds of amino acid as same as in Ref.[26].